W. Nuhsbaum, Inc. 760 Ridgeview DriveMcHenry, IL60050 Phone: (800)-368-3368/(815) 385-5200  Fax: (815)-385-5236

 

CARV II IMAGE ANALYSIS SYSTEM

1- CCD Detector:  A wide range of application specific CCD cameras can be used with the confocal device including: Cooke (Sensicam QE, Sensicam EM), Roper (CoolSNAP™ ES, CoolSNAP™ HQ, Cascade™), Hamamatsu® (ORCA ER, AG), and more. 2 - Light Source:   A long life Hg/metal halide arc source, coupled to the instrument via an alignment-free light guide, allows for full spectrum (360 nm – 700 nm) confocal imaging of virtually any fluorescent probe. 3 - Easily Switch Between Confocal and Widefield Imaging/Viewing Modes:    The CARV II Confocal Imager permits direct viewing of confocal images through a binocular eyepiece and/or through the camera for fast imaging setup. It is the only pinhole spinning disk fluorescence confocal systems which allows the user to quickly switch from confocal to wide-field viewing or recording. 4 - Automated Filter Changers:  Automation of internal multi-position excitation (8 position), dichroic (5 position) and emission (8 position) filter wheels allows fast multi-dimensional confocal imaging.  The automated filters reduce the reliance on multi-band pass filter sets allowing maximum light throughput and fast sequential imaging of up to five or more fluorescent probes in the same sample. 5 - FRAP Capabilities: A FRAP slit at the same focal plane as the confocal disk creates an adjustable rectangular aperture on the image.  Passing high-intensity Hg/metal halide light through this slit enables controlled photobleaching of part of the sample followed by fluorescence recovery recording.  6- Microscope Compatibility: The CARV II Confocal Imager unit can be configured to most inverted and upright fluorescent microscopes converting your existing fluorescence microscope to a confocal imager. 7 - Imaging Software: A range of state of the art 3D software packages can be used for acquisition and analysis of confocal images including IPLab (Scanalytics) and Metamorph (Molecular Devices).  Command sets needed to control the CARV II are available on request.

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